Figures and table legends
Figure 1. The Kungsängen Nature Reserve field site is
located a) south of the city of Uppsala, in central Sweden (red circle).
b) It is part of a large meadow on the east side of the Fyris river, a
green line indicating reserve borders. Red dots indicate soil sampling
locations on either side of the soil moisture transition border (red
line) intersecting two permanent plant community transects T1 and T4
(dashed lines). Map source: © Lantmäteriet, i2012/921.
Figure 2. Non-metric multidimensional scaling ordination (nMDS)
of the plant community in plots along transect T1 (plots 1-28, red) and
T4 (plots 61-76 blue). Ellipses outline the distribution of plots from
the wet part (green) and mesic-dry part (black). Plots 75 and 76 are on
the levee close to the river with somewhat deviating vegetation. The
closest plots to the locations for soil microbiome sampling (enclosed by
dashed ellipses) on the mesic-dry side were plots 14-16 (T1) and 67-68
(T4) and on the wet side plots 17-18 (T1) and 69 (T4).
Figure 3 . Non-metric multidimensional scaling (nMDS) ordination
of microeukaryotic communities recovered from wet (green) and mesic-dry
(red) soil moisture regimes at the Kungsängen Nature Reserve using
Bray-Curtis dissimilarities calculated from relative abundance based on
three different OTU inference methods a) OTU_A, b) OTU_S and c)
OTU_C. Closed circles are samples with a F. meleagris plant and
open triangles are samples without F. meleagris plant
Figure 4. Phylum-level taxonomic assignments of microeukaryotic
communities in wet and mesic-dry soil moisture regimes separated into
(A) protists and (B) Fungi. Illustrated as mean fractional read
abundance for the three occurrence tables OTU_A, OTU_C and OTU_S.
Phyla which represent less than 1.5% of total reads are grouped
together as “other”.
Figure 5. Species richness curve estimated from the bottom up
as SH_99, SH_97 and GH_90, based on all reads combined for ten
samples each from mesic-dry and wet soil moisture regies. OTUs were
inferred using three different clustering methods: OTU_A (green),
OTU_C (orange) and OTU_S (purple) and the ITS2 region of their
representative sequences were then clustered into species and genus
hypotheses (SH and GH), using three different ITS2 sequence similarity
thresholds 99% for SH_99, 97% for SH_97 and 90% for GH_90.
Table 1. Number of inferred OTUs and the number of reads
represented, for total microeukaryotic community and (fungi), for the
three different clustering methods.
Table 2. OTU sequences, were clustered across the ITS2 region,
to represent taxa at different taxonomic ranks genus (GH_90) and
species (SH_97 and SH_99), number of clusters and % of these
including sequences from the three OTU inference methods.