OTU accumulation curves
OTU accumulation curves and asymptotic species richness estimates were calculated using the iNEXT package (Hsieh et al., 2016) in R (version 4.0.4; R Core Team, 2019). For this analysis, accumulation curves for OTUs, SH_99, SH_97 or GH_90 were calculated separately for the three clustering methods, the two different soil moisture regimes (wet and mesic-dry) based on the number of raw reads obtained in each soil moisture regime and the number of samples.