Figure 2 Dimerization of ppnP is conserved in bacteria.(A ) In solution validation the aggregation status of theEc ppnP by gel filtration analysis. (B ) SDS-PAGE showed that the double mutant V27D/V29D of Ec ppnP is not stable. (C-E ) The dimeric structural comparison of Ec ppnP toPa ppnP, St ppnP, and Vc ppnP. The Pa ppnP is colored green, the St ppnP is colored in purple-blue, and theVc ppnP is colored in teal.