2.2 Genome size assessment
The clean reads (152 Gb) from the 350 bp insert-size Illumina library
were used to estimate the genome size based on the 17-mer method.
Jellyfish
(Marçais & Kingsford, 2011) was used to generate the k -mer
frequency distribution. Genomescope (Ranallo-Benavidez, Jaron, &
Schatz, 2020) was employed to estimate the genome size. The k -mer
number was 33,414,462,943, with a peak depth of 34. The estimated genome
size of S. tetraptera was ~ 982.78 Mb (Figure S1,
Table S1).