Ex vivo thrombus growth analysis
Ex vivo thrombus growth analysis was conducted as described previously 27,31. Briefly, hirudinated whole blood was preincubated with DiOC-6 (500 nM) and perfused through the parallel plate flow chambers with fibrillary collagen (100 µg/mL) covered glass at 100 s-1 shear rate. Thrombus growth was observed using a Nikon Eclipse Ti-E inverted microscope (Tokyo, Japan).