Ex vivo thrombus growth analysis
Ex vivo thrombus growth analysis was conducted as described
previously 27,31. Briefly, hirudinated whole blood was
preincubated with DiOC-6 (500 nM) and perfused through the parallel
plate flow chambers with fibrillary collagen (100 µg/mL) covered glass
at 100 s-1 shear rate. Thrombus growth was observed
using a Nikon Eclipse Ti-E inverted microscope (Tokyo, Japan).