Materials and methods
Yeast strains and
stocks
Yarrowia lipolytica W29 used in this study is a natural strain,
originally isolated from wastewater in Paris, France. TheSaccharomyces cerevisiae strains used in this study share the
CEN.PK genetic background (Entian and Kötter, 2007; Salazar et
al. , 2017) and the FDH knockout strain CEN.PK556-7B was constructed
previously (Overkamp et al. , 2002). Frozen culture stocks were
prepared by adding sterile glycerol to an overnight culture to a final
glycerol concentration of 30% v/v and storage of 1 mL aliquots at
-80°C.