Conventional Echocardiogram
The echocardiogram was performed by a single operator blind to the allocation group. Commercially available echocardiographic equipment (Vivid E9; GE Medical Systems, Milwaukee, WI, USA) was used, equipped with linear broadband transducers with a frequency of 5-2 MHz. Cavity dimensions were measured from longitudinal parasternal view. The systolic and diastolic cavity volumes, and LVEF, were calculated using Simpson’s modified biplanar method, as recommended by the American Society of Echocardiography.27 The LV segmental contraction analysis was performed through wall motion score index calculation (WMSI). Valve flow analysis and LV diastolic function were was performed according to current guidelines.28-30