Phytohormones analysis
Fresh maize leaves were cut and transferred into liquid nitrogen
immediately. The samples 0.4 g were completely ground and 1 mL of a
mixture solution (methanol: formic acid: water=15:1:4, v/v/v) was added
for extraction. Purification was done using the dual-mode solid-phase
extraction method (Dobrev & KamĂnek,
2002). Analysis of endogenous phytohormones, abscisic acid (ABA), Auxin
(IAA), and gibberellin (GA) was done according to a previously described
method (Meng et al., 2016). In addition,
ethylene measurement is described earlier
(Murad et al., 2021;
Zaidi et al., 2016).