Sequencing and Taxonomy
Following sample processing, sequencing, and quality control, a total of
1,740 16S rRNA amplicon sequence libraries were generated, with an
average read depth of 65,595 reads per library (standard devion
[SD]. +/- 47,441 reads). A total of 1,606 libraries were retained
for analyses following rarefaction (Supplemental Table S1). All
subsequent results were derived from rarefied data. Taxonomic
composition across all body sites was dominated by Proteobacteria and
Firmicutes, although relative abundance of these and other phyla
differed between body sites (Fig. 2, Supplemental Table S2).