Fig. 2. Metabolic pathway engineering for TAL biosynthesis inR. toruloides . PDC1, pyruvate decarboxylase; ALD5, acetylaldehyde dehydrogenase; ACS1, acetyl-CoA synthetase ACS1, ACC1, acetyl-CoA carboxylase; GhPS, 2-pyrone synthase gene from Gerbera hybrida ; PDH, pyruvate dehydrogenase; ACL1, citrate lyase; YLACL1, citrate lyase from Yarrowia lipolytica ; AMPD1, AMP deaminase; ME1, malic enzyme; PEX10, peroxisomal matrix protein; PYC1, pyruvate carboxylase; MLS1, cytosolic malate synthase; CIT2, peroxisomal citrate synthase; DGA1, diacylglycerol acyltransferase; LRO1, lecithin cholesterol acyltransferase. Note: Some metabolites were not positioned following their intracellular compartmentalization.