pRS426 |
Used for cloning of pMB1 origin, AmpR,
and S. cerevisiae elements of 2µ origin and URA3
|
Laboratory stock |
pGI2 |
KanR for bacteria,
NatR for yeast, binary plasmid |
(Abbott et
al., 2013) |
pGI2_880_ACC |
Used for cloning of ACC1 gene from R.
toruloides
|
(Zhang et al., 2016) |
pGI2-TEF1-X |
Expression vector in R. toruloides,
containing NatR, pTEF1 promoter and
T35S terminator |
This study |
pRTN |
Expression vector in R. toruloides, containing
NatR, pANT promoter and T35S
terminator |
Laboratory stock |
pRTG2-X |
Derived from pRTN, containing G418R,
pANT promoter and T35S terminator |
This
study |
pRTHyg-X |
Derived from pRTN, containing HygR,
pANT promoter and T35S terminator |
This
study |
pRTH |
gRNA expression vector in R. toruloides, containing
HygR, 5S-tRNATyr, 2
BsaI sites, and SUP4 terminator for SgRNA |
Laboratory
stock |
pRTH-X |
Derived from pRTH with target N20 insertion at BsaI
sites (no BsaI left) |
This study |
pRT-Cas9-SgRNA-Hyg |
Derived from pRTH-X with Cas9 expression
cassette |
This study |
Strains |
Description |
Source |
DH5α |
Cloning host |
NEB |
IFO0880 |
R. toruloides strain IFO0880, mating type A2 |
NBRC,
Japan |
I12 |
IFO0880 with genome integrated codon optimized GhPS
cassette |
This study |
I12-Cas9
|
I12 with genome integrated codon optimized Cas9
cassette |
This study |
I12-ACL1
|
I12 with genome integrated ACL1 cassette |
This
study |
I12-ACL1-ACC1
|
I12-ACL1 with genome integrated
ACC1 cassette |
This study |