Western Blot analysis of alpha-L-iduronidase expressing B. rapa rapa hairy roots
Western Blot analysis of alpha-L-iduronidase (IDUA) samples (crude culture media) was performed as previously described (Cardon et al., 2019) with the exception that after the mouse IDUA-binding primary antibody (ABIN603316 from Antibodies-online), the subsequent secondary antibody used was the IRDye 680RD Goat anti-mouse (926-68070 from Li-cor ). Scanning of the blots after incubation and washing was performed with the Odyssey CLx Imaging System. Relative densitometries were analyzed using Image Studio Lite Ver 5.2.