CONCLUSIONS
Hairy root cultures represent a promising, scalable manufacturing system for high-value biopharmaceuticals. In order to maximize the productivity a careful optimization of the whole production process is required, including an optimization both of the culture medium used for hairy root growth and of the culture medium used during the recombinant protein production phase of the hairy root system. We studied suitability ofBrassica rapa rapa hairy roots for secretion of a biopharmaceutical, namely α-L-iduronidase (IDUA). Our data first showed that with careful process optimization high level of secretion of a model protein such as eGFP can be obtained. We also demonstrated that such optimization can also allow to increase the ability of hairy roots to produce and secrete complex proteins. Thus Brassica rapa rapahairy roots hold high potential as a production platform for complex human glycoproteins such as IDUA and indeed also for other biologics.