Figure 7. Effect of isorhamnetin based on western botting and RT-PCR findings.
(7.1A-C) Quantification of the levels of Camkii , Ryr2 , andCacna1c . qRT-PCR analysis of mRNA levels normalized to 18 s (control, n = 6; AngII, n = 6; AngII+isorhamnetin, n = 6). (7.1D) Representative images obtained from western blotting using atrial tissue, and western blot analysis with ox-CAMKII, p-RyR2 (Ser2814), TRPC3, TRPC6, and CACNA1C. (7.1E-I) Quantification of intensity analyzed using western blot images (control, n = 6; Ang II, n = 6; AngII+isorhamnetin, n = 6). **P < 0.01 control vs. AngII; *P < 0.05 control vs. AngII by one-way ANOVA with post-hoc test (Tukey); ##P< 0.01 AngII vs. AngII+isorhamnetin;#P < 0.05 AngII vs. AngII+isorhamnetin by one-way ANOVA with post-hoc test (Tukey). (7.2A) Representative images obtained by western blotting of atrial tissue. Western blot analysis with p-JNK, JNK, p-ERK, ERK, p-NF-κB, and GAPDH. (7.2B-D) Quantification of intensity analyzed by western blot images (control, n = 6; AngII, n = 6; AngII+isorhamnetin, n = 6). **P < 0.01 control vs. AngII by one-way ANOVA withpost-hoc test (Tukey). ##P < 0.01 AngII vs. AngII+isorhamnetin; #P< 0.05 AngII vs. AngII+isorhamnetin by one-way ANOVA withpost-hoc test (Tukey).