3.3 Isorhamnetin prevented AngII-induced abnormal diastolic
intracellular Ca2+ loading in HL-1 cells
HL-1 cells can self-generate spontaneous potentials without electrical
stimuli; therefore, spontaneous Ca2+ waves can be
observed with the use of Fluo-4 (Fig. 3A). The Ca2+amplitude (F/F0) was significantly higher in the AngII
treatment group than in the isorhamnetin treatment group (Fig. 3C). In
addition, AngII shortened the time to peak and decay time. In contrast,
shortened time to peak and decay time were reversed by isorhamnetin
treatment (Fig. 3D-E). Furthermore, during the depolarization process,
AngII increased the occurrence of spontaneous abnormal
Ca2+ waves more frequently than in the control, while
isorhamnetin treatment suppressed them (Fig. 3A, G). These cellular
electrophysiology results suggest that isorhamnetin reversed
AngII-induced abnormal Ca2+ loading and aberrant
diastolic Ca2+ activity.