Figure 2. Isorhamnetin inhibited AngII-induced diastolic abnormal Ca2+ activities in atrial myocyte.
(A) Representative confocal line scan images showing Ca2+ sparks in atrial myocytes isolated from three groups under stimulation with Isoproterenol (1 µM). (B) Quantification of Ca2+ spark frequency (CaSpF) analyzed using the spark master plugin on ImageJ software. (C) Quantification of Ca2+ amplitude (F-F0/F0) analyzed using ImageJ software (control, n = 23 cells from 5 mice; AngII, n = 29 cells from 5 mice; AngII+isorhamnetin, n = 21 cells from 5 mice). **P < 0.01 control vs. AngII by one-way ANOVA with post-hoc test (Tukey); ##P < 0.01 AngII vs. AngII+isorhamnetin by one-way ANOVA with post-hoc test (Tukey).