3.4 Isorhamnetin reversed AngII-induced APD prolongation in Hl-1 cells
Alterations in ionic currents can change the action potential (AP) morphology and cause abnormal depolarization (Jansen et al., 2019). Additionally, DADs are known to trigger abnormal AP (Tse, 2016). To clarify the effect of isorhamnetin on APD, AP morphology was captured using MEA in HL-1 cells. The AngII group had remarkably prolonged APD20, APD50, and APD90. However, isorhamnetin reversed APD prolongation at all time points caused by AngII (Fig. 4G, H-J). Moreover, during the burst pacing process, DADs appeared in the AngII treatment group, and after pacing, aberrant spontaneous potentials were also observed in the AngII treatment group, whereas isorhamnetin diminished DADs (Fig. 4B, K). Taken together, isorhamnetin normalized the AP morphology and abrogated the occurrence of DADs.