2.9 Statistical analysis
The data and statistical analysis comply with the recommendations of the British Journal of Pharmacology on experimental design and analysis in pharmacology (Curtis et al., 2018). Data and statistical analyses were performed with a group size of n ≥ 5. The group size (n) is listed in the figure legends, respectively, and refer to the number of independent values used in the statistical analysis. The results are presented as the means ± SEM without outlier removal. All data were tested for normality with the Shapiro-Wilk test, and statistical comparisons between the three groups were conducted using one-way analysis of variance with a post-hoc test (Tukey) for comparison of groups with normal distribution of data. Differences were considered statistically significant at P < 0.05. All statistical analyses were performed using GraphPad Prism (version 9, GraphPad Software, San Diego, CA, USA).