2.9 Statistical analysis
The data and statistical analysis comply with the recommendations of the
British Journal of Pharmacology on experimental design and analysis in
pharmacology (Curtis et al., 2018). Data and statistical analyses were
performed with a group size of n ≥ 5. The group size (n) is listed in
the figure legends, respectively, and refer to the number of independent
values used in the statistical analysis. The results are presented as
the means ± SEM without outlier removal. All data were tested for
normality with the Shapiro-Wilk test, and statistical comparisons
between the three groups were conducted using one-way analysis of
variance with a post-hoc test (Tukey) for comparison of groups
with normal distribution of data. Differences were considered
statistically significant at P < 0.05. All statistical
analyses were performed using GraphPad Prism (version 9, GraphPad
Software, San Diego, CA, USA).