4.8 Catechol oxidation assay
CTRL and EXP group bacteria were collected by centrifugation of
5,000-8,000 r.p.m for 2 minutes and washed twice using PBS. Then, 100 μL
PBS culture were added to each well of 96-well plate, and the mCherry
fluorescence level of EXP groups and CTRL groups were matched to each
other. Prior to each assay, 100 mM catechol water solution was diluted
to 10 mM working solution, 2.5 μL of which was then added to each well.
The absorbance of oxidation product 2-HMS at 377 nm can be measured at
19-22℃. Catechol was purchased from Damas-beta (120-80-9). Thermo
Scientific Varioskan LUX was used for quantitating reactions. SkanIt
software was used for data management.
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Figure legend