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\begin{document}

\title{EXPLORING METABOLIC AND STOICHIOMETRIC CONTROLS FOR NUTRIENT EXCRETION:
BODY SIZE HAS PRIMACY IN A TROPICAL STREAM FISH COMMUNITY}



\author[1]{Priscila Oliveira-Cunha}%
\author[2]{Peter B. McIntyre}%
\author[1]{Vinicius Neres-Lima}%
\author[3]{Adriano Caliman}%
\author[1]{Beatriz Moreira-Ferreira}%
\author[1]{Eugenia Zandona}%
\affil[1]{Universidade do Estado do Rio de Janeiro}%
\affil[2]{Cornell University}%
\affil[3]{Federal University of Rio Grande do Norte}%


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  \date{May 29, 2024}


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\begin{abstract}
Discussions of the factors regulating nutrient recycling by consumers
have focused on predictions from Ecological Stoichiometry (ES) and the
Metabolic Theory of Ecology (MTE). ES posits that imbalances between the
composition of an animal's body tissues and its diet should determine
its nutrient excretion rates, whereas the MTE predicts that excretion
should directly reflect metabolic activity arising from body size and
temperature. Each framework has been supported by data, but they are
rarely tested together. In this study, we measured excretion rates of
nitrogen (NH4), phosphorus (SRP) and N:P excretion ratio, body N:P
stoichiometry, body size, and temperature for 12 species of fish from an
Atlantic rainforest stream in Brazil. We fitted 8 competing models
reflecting different combinations of ES (body N:P, armor classification,
diet group) and MTE (body size, temperature) variables. For both N and P
excretion, as well as excreted N:P ratio, only body size was included in
the best model, and interspecific differences in size-scaling were
greater for N than for P. Fitted size scaling coefficients were lower
than the MTE prediction of 0.75 for both N (0.59, 95\% CI = 0.45, 0.73)
and P (0.56, 95\% CI = 0.40, 0.77). There was only weak evidence that
body armor in 3 of 12 species led to more retention of P, and there was
no discernable effect of diet group, body N:P, or water temperature. We
conclude that differences in nutrient excretion among species within a
shared environment primarily reflect contrasts in metabolic rates
arising from body size, rather than disparities between consumer and
resource stoichiometry. Our findings align with those from other
ecosystems and synthesis across aquatic taxa, expanding support for the
MTE as the primary framework for predicting nutrient excretion rates.
Key words: ecological stoichiometry, metabolic ecology, animals,
nitrogen, phosphorus, freshwater.%
\end{abstract}%



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