CDC42, S100A9 and Ficolin-2 are increased in AnEVs
Three proteins (CDC42, S100A9 and Ficolin-2) were selected in order to mainly confirmation of the massive previous identification. By differential proteomic analysis, we observed that the abundance of CDC42, S100A9 and Ficolin-2 was increased in AnEVs. Therefore, we extended the isolation of plasma circulating EVs to a larger cohort of samples obtained in basal conditions and during the acute phase of the anaphylactic reaction. The specific analysis for CDC42, S100A9 and Ficolin-2 demonstrated an increase of these proteins in AnEVs, which agreed with previous observed data (Figure 3A-B ).