Proteomic profiling of AnEVs and BEVs
To investigate the protein pattern profile of plasma EVs in anaphylaxis,
10 acute and baseline paired samples were analyzed by RP-LC-MS/MS and
the PL relative quantification method identified 1206 proteins. When
comparing brute values of AnEVs and BEVs, we found 526 proteins
exclusively detected in EVs released during the acute phase of the
reaction (Figure 2A ). Volcano plot depicts the whole amount of
proteins identified, in which we observed different abundance of some
markers of interest (Figure 2B ). Principal component analysis
(PCA) revealed grouping of biological replicates and separation of BEV
and AnEVs groups by PC1 (Figure 2C ). Statistical analysis
revealed 99 differentially expressed EVs proteins. 83 were enriched in
the acute phase, and 16 were more abundant in basal conditions
(Supplemental Table 1 ). The profile of these proteins shows how
different AnEv samples cluster together and clearly differentiated from
BEVs (Figure 2D ).