Characterization of EVs from anaphylactic patients
AnEVs and BEVs were both isolated and characterized using different
techniques. Electron microscopy showed 100-250nm particles consistent
with EVs reported size range [27] (Figure 1A ). These
results were confirmed by NTA, revealing heterogeneous particle
populations (Figure 1B ). The analysis showed a 200nm peak,
confirming EVs enrichment in our preparation. Furthermore, no
differences were observed in particle number or concentration between
BEVs and AnEVs (Figure 1C-D ). In addition, we confirmed EV
purification by immunodetection of TSG101, CD9, CD63 and Syntenin-1, all
of them considered bona fide EVs protein markers (Figure
1E ).