METHODS
An expanded Materials and Methods Section is available in the Online Repository Material.
Patients’ selection
The studied population included 86 paired peripheral blood samples (plasma) from 43 patients presenting anaphylaxis and recruited at Fundación Jiménez Díaz and Hospital Central de la Cruz Roja, Madrid, Spain. From every subject, EVs were purified from plasma collected in the acute phase of the episode (AnEVs) and at baseline (BEVs) at least 14 days after reaction Patients who fulfilled the definition of anaphylaxis according to the 2006 National Institute of Allergy and Infectious Disease/Food Allergy and Anaphylaxis Network criteria were included [2]. The protocol was approved by the Ethics Committee (PIC38/2016_FJD and PIC142/2016_FJD).