Characteristics of the cohort
The studied population included a sum of 86 plasma samples from 43 anaphylactic patients ranged in age from 20-76 years old (45.2±2.3) and 65% were female. Triggers suspected to provoke reactions were drugs (67%), food (19%) and others (14%). The details of each individual case (criteria of severity) were applied based on clinical symptoms and according to the grading system established in Brown classification [25]. The mild group was grade 1 (12% of the population), moderate was grade 2 (56%), and severe was grade 3 (32%). This last classifies severe anaphylaxis with cardiovascular involvement and hypotension. The percentage of administered drugs was: epinephrine (53%), β2-adrenergic agonist (14%), histamine receptor antagonist type 1 (65%) and 2 (21%) and steroids (67%). In addition, 39% of patients presented elevated tryptase (>11.4µg/l) [26] in the acute phase with a medium value of 11.8±1.8µg/l in our group. A fulfilled clinical description and demographic characteristics of the anaphylactic population are showed in Table 1.