Statistical Analysis
Statistical analysis was carried out with the Graph Pad Prism 8 program (La Jolla, CA, USA) using Student’s t analysis to determine the significant differences between the 2 groups and the analysis of variances 2-way ANOVA and Bonferroni’s multiple comparisons test when compared more than 2. All values are represented with their mean ± standard error of the mean (SEM) and the differences were considered significant at a level of p value <0.05.