Fig. 5. GS increase NAD+-dependent activation
of SIRT1 in drosophila and mice. (A) The NAD+level and NAD+/NADH ratio in the brain tissue of 10
flies from control and GS treatment groups were analyzed by a
bioluminescent detection kit. (B) The dSir2 (SIRT1) level in
the drosophila brain of control and
GS groups was detected by Western blot and quantified as the relative
expression on the right. JLA20 is the loading control. (C) The
activities and climbing distance of 30 flies per group were investigated
after treatment with GS at 1 mg/mL for 7 d. (D) IHC staining of
SIRT1 in the heart and brain tissues from control and GS-treated mice
(10 mg/kg, 21 days) was performed. Scale bar=50 μm. Ctrl: control group.
*P < 0.05, **P < 0.01, and ***P< 0.001 versus Ctrl group.