Fig. 2. Effect of GS on key metabolites and enzymes of glucose metabolism in H9c2 and HUVECs.(A) The heatmap represents the targeted metabolomic analysis of 15 key metabolites of glucose metabolism in control and GS-pretreated H9c2 cells, after 48 h incubation.(B) Bar graph depicts the quantification of 15 metabolites in H9c2 cells in control and GS-pretreated H9c2 cells. (C) The heatmap showing 15 metabolites of glucose metabolism in control and GS-pretreated HUVECs.(D) After GS pretreatment for 48 h, key enzymes of glycolysis and TCA cycle and mitochondrial pyruvate carriers were detected by Western blot analysis. (E) Pyruvate dehydrogenase (PDH) activities in H9c2 and HUVECs cells pretreated with GS for 48 h were measured. Ctrl: control group. *P < 0.05, **P< 0.01, and ***P < 0.001 versus Ctrl group.