Measurement of PDH activity
PDH activity was measured using a commercial assay kit (ab109902; Abcam), according to the manufacturer’s instruction (Baccelli et al., 2019). Briefly, cell lysates from different groups were loaded onto the 96-well plates coated with an anti-PDH monoclonal antibody and incubated for 3 h at room temperature for the measurement of optical density at 450 nm (OD 450 nm) for 30 min with 1 min internal in a kinetic mode. The relative PDH activity was calculated as the rate (mOD/min) = (absorbance 2-absorbance 1)/time (min).