Fig. 2. Effect of GS on key metabolites and enzymes of
glucose metabolism in H9c2 and
HUVECs.(A) The heatmap represents the targeted metabolomic analysis of
15 key metabolites of glucose metabolism in control and GS-pretreated
H9c2 cells, after 48 h incubation.(B) Bar graph depicts the
quantification of 15 metabolites in H9c2 cells in control and
GS-pretreated H9c2 cells. (C) The heatmap showing 15
metabolites of glucose metabolism in control and GS-pretreated HUVECs.(D) After GS pretreatment for 48 h, key enzymes of glycolysis
and TCA cycle and mitochondrial pyruvate carriers were detected by
Western blot analysis. (E) Pyruvate dehydrogenase (PDH)
activities in H9c2 and HUVECs cells pretreated with GS for 48 h were
measured. Ctrl: control group. *P < 0.05, **P< 0.01, and ***P < 0.001 versus Ctrl
group.