Fig. 5. GS increase NAD+-dependent activation of SIRT1 in drosophila and mice. (A) The NAD+level and NAD+/NADH ratio in the brain tissue of 10 flies from control and GS treatment groups were analyzed by a bioluminescent detection kit. (B) The dSir2 (SIRT1) level in the drosophila brain of control and GS groups was detected by Western blot and quantified as the relative expression on the right. JLA20 is the loading control. (C) The activities and climbing distance of 30 flies per group were investigated after treatment with GS at 1 mg/mL for 7 d. (D) IHC staining of SIRT1 in the heart and brain tissues from control and GS-treated mice (10 mg/kg, 21 days) was performed. Scale bar=50 μm. Ctrl: control group. *P < 0.05, **P < 0.01, and ***P< 0.001 versus Ctrl group.