Fig. 8. The effects of main ginsenosides monomers on SIRT1-mediated mitochondrial function in H9c2 cells. (A-B)Basal OCR and ATP production were detected in H9c2 cells treated with GS or different ginsenoside monomer for 48 h as described in Materials and Methods. (C-D) After the pretreatment with different ginsenosides monomers at the composition percentage of GS (5μg/mL) for 48 h, the levels of HK-II, MPC1 and SIRT1 in H9c2 cells were detected by Western blot. Relative HK-II, MPC1, and SIRT1 expressions were quantified after the normalization to β-Actin. β-Actin is the loading control for Western blot analysis. Ctrl: control group, OCR: oxygen consumption rate. HK-II: Hexokinase-II, MPC1: mitochondrial pyruvate carrier 1. *P < 0.05, **P < 0.01, and ***P < 0.001 versus Ctrl group.