Measurement of PDH activity
PDH activity was measured using a commercial assay kit
(ab109902;
Abcam), according to the manufacturer’s instruction
(Baccelli et al., 2019). Briefly, cell
lysates from different groups were loaded onto the 96-well plates coated
with an anti-PDH monoclonal antibody and incubated for 3 h at room
temperature for the measurement of optical density at 450 nm (OD 450 nm)
for 30 min with 1 min internal in a kinetic mode. The relative PDH
activity was calculated as the rate (mOD/min) = (absorbance 2-absorbance
1)/time (min).