Figure legends
Figure 1. (a) Initial CT images of the primary lesion replacing
the thyroid lobe. (b) Representative CT image of the liver metastasis
after lenvatinib treatment. Red arrows indicate the tumor lesions. (c)
Low- (upper) and high- (lower) magnification of HE-stained images of the
metastatic ITTC. (d) Immunohistochemical staining of CD5, p40, PAX5, and
TTF-1 in the metastatic ITTC.
Figure 2. Low- (left) and high- (right) magnification images of
PD-L1 immunohistochemical staining on tumor cells within the metastatic
ITTC.
Figure 3. (a) Left: separation of different tumor areas on
representative image of the metastatic ITTC indicated by block dashed
lines. Contour plots of PD-L1+ tumor cells (middle)
and PD-1+ T cells (right). (b) Representative HE
staining (left) and multiplex IHC (right) images of the metastatic ITTC.
Biomarkers and colors are shown on the right side. (c) Gating strategy
of PD-1+CD3+CD8+ T
cells shown in the image cytometry plots. Subject image is in figure 3a.
(d) Frequencies of PD-1-expressing
CD3+CD8+ T cells in different tumor
areas.