3.3. SDS-PAGE analysis
As shown in Fig. 2D, ORF8 (Lane 2) and CRM197 (Lane 3) both exhibited a single band corresponding to the Mw values of 15 kDa and 59 kDa, respectively. In contrast, ORF8-CRM (Lane 4) displayed a single electrophoresis band with slow mobility, corresponding to an Mw of more than 160 kDa. ORF8-PEG (Lane 5) exhibited a disperse band corresponding to an Mw of 100-200 kDa.