3.9. Toxicity study
CK and LDH indicated the cardiac function; ALT, TP and ALB indicated the hepatic function; UA indicated the renal function. The potential toxicity of ORF8-CRM and ORF-PEG to the cardiac, liver and renal functions of mice was evaluated by measuring these substances in the mice sera. As shown in Table 1, the PBS group displayed certain levels of these substances. As compared with the PBS group, the ORF8 group exhibited no significant differences in these parameters, except a slightly high ALB level; the ORF8-CRM and ORF8/AL groups exhibited no apparent differences except a slightly low LDH and CK levels. These parameters of the ORF8-PEG group were comparable to those of the PBS group. Thus, ORF8-CRM and ORF8-PEG showed no apparent toxicity to the cardiac, liver and renal functions of mice.