2.14. Toxicity study
Biochemical analysis of the mouse sera was carried out to evaluate the potential toxicity of the samples to the cardiac, hepatic, and renal functions. The serum samples of the five groups were collected on day 21 for toxicity study. Creatine kinase (CK) and lactate dehydrogenase (LDH) in the sera were determined for cardiac function. Alanine aminotransferase (ALT), total protein (TP) and albumin (ALB) were measured for hepatic function. Uric acid (UA) and were assayed for renal function. All analyses were carried out using an Analyzer Medical System (AUTO LAB, Italy).