2.7. Size exclusion chromatography
The samples were analyzed by size exclusion chromatography (SEC), using
an analytical Superdex 200 column (1 cm × 30 cm, GE Healthcare, USA).
The column was equilibrated and eluted by PBS buffer (pH 7.4) at a
constant flow rate of 0.5 mL/min. The effluent was detected at 280 nm.