RNA isolation and qRT-PCR.
Total RNA was isolated using a Promega RNA isolation kit (Eastep®
Super), then cDNA was synthesized using a CWBIO kit (CW0690L). The
real-time quantitative PCR was performed by the quantitative
fluorescence kit (SYBR® Premix Ex TaqTM Ⅱ, Takara, Kyoto, Japan) using a
Real-time PCR detection system in Bio-Rad’s ICYCleriQS fluorescent
quantitative PCR instrument. The nucleotide sequences of the primer
pairs used for qRT-PCR and cloning are shown in Supplementary Table 1.