Figure 9. Disruption of BnPHT5;1b affects seeds traits of Brassica napus grown under NP and HP conditions.
(A) Seed size of W10 and BnPHT5;1b double mutants. Scar bar= 1 cm. (B) Seed diameter; (C) 1000-seed weight;(D) P concentration of seeds; (E) seeds germination rate. Seeds were incubated 6 days in pure water for germination assay. Data are mean ± SD (n=6). NP, normal P supply, 150 mg P/kg soil; HP, high P supply, 500 mg P/kg soil. BnPHT5;1b DM #25 andBnPHT5;1b DM #31 , BnPHT5;1b double mutants. Asterisks indicate significant differences from control (Student’s t-test, * P < 0.05, ** P < 0. 01, *** P < 0.001).