Figure 8. Disruption of BnPHT5;1b causes excessive allocation of Pi to the flowers of Brassica napus and the reduction of seed yield and silique number.
(a) qRT-PCR analysis of BnPHT5;1b expression in various tissues of W10 grown in NP conditions at the flowering stage.BnEF-α and BnTubulin mRNAs were used as internal controls. Data are shown as the mean ± SD (n = 4). (b-d) Comparison of total P concentrations in the shoot (stem, leaf and flowers)(b) and Pi concentrations in floral organs (c) between W10 and BnPHT5;1b double mutants grown in NP conditions and total P concentrations in shoot (stem, leaf and flowers) between W10 andBnPHT5;1b double mutants under HP conditions (d ). Data are mean ± SD (n=5). Asterisks indicate significant differences from control (Student’s t-test, * P < 0.05, ** P < 0. 01, *** P < 0.001). (e, f) The silique number and seed yield of W10 and BnPHT5;1b double mutants grown under NP and HP conditions. Data are mean ± SD (n=6). Different letters above columns indicate a significant difference among different lines under each P treatment (P < 0.05 by Tukey’s test). NP, normal P supply, 150 mg P/kg soil; HP, high P supply, 500 mg P/kg soil. BnPHT5;1b DM #25 and BnPHT5;1b DM #31 , BnPHT5;1b double mutants.