Figure 2. Expression of BnPHT5;1bs in Brassica napus and Arabidopsis organs.
(a) The relative expression of BnPHT5;1bs in different organs of Brassica napus . 16-day-old Brassica napusseedlings grown in hydroponic medium supplemented with 5 μM, 100 μM and 1000 μM P were used. Mean values ±SD are shown (n = 3). Different lowercase letters above columns indicate significant differences among organs of Brassica napus cultivar Westar 10 under the same P treatment (P < 0.05 by Tukey’s test). OL, old leaf; ML, mature leaf; YL, young leaf. (b, c) GUS staining of ProBnA09PHT5;1b :GUS and ProBnCnPHT5;1b :GUS transgenic Arabidopsis (Col-0) plants grown at 100 μM and 1,000 μM P supply. 10-day-old seedlings were used. Bars = 100 μm.