Figure 1. Identification of the PHT5 family of proteins inBrassica napus and their transcriptional response to Pi supply.
(a) The PHT5 family in Brassica napus andArabidopsis . The phylogenetic tree was generated by MEGA 6 using the protein sequence of AtPHT5 family members and BnPHT5 family members with the neighbor-joining (NJ) method and 1000 bootstrap replicates. (b-g) qRT-PCR analysis of the expression of theBnPHT5 family in leaves grown at different phosphorus supplies. The relative expression level of BnPHT5;1a (b, e) ,BnPHT5;1b (c, f) and BnPHT5;3 (d, g ) inBrassica napus cultivar Westar 10 grown with different phosphorus concentrations in a hydroponic medium (0 μM, 5 μM, 25 μM, 50 μM and 100 μM P supply). 9-day-old Brassica napus seedlings were used for mRNA extraction. Mean values ± SD are shown (n = 3). Different lowercase letters above columns indicate a significant difference in relative expression level of a gene among different P treatments (P < 0.05 by Tukey’s test).