Figure 7. Disruption of BnPHT5;1b reduces Brassica napus adaptability to fluctuations in Pi supply.
(a) The phenotype of W10 and BnPHT5;1b DM lines. 5-day-old seedlings grown under different Pi supplies (100 µM, 1,000 µM, 2,000 µM) were transferred to media lacking Pi for 14 days. Scale bars = 5 cm. (b) Biomass, (c) Pi concentration and(d) leaf anthocyanin concentration of W10 and BnPHT5;1bdouble mutants following changes in Pi supply. W10, Westar 10, wild type; BnPHT5;1b DM #25 and BnPHT5;1b DM #31 ,BnPHT5;1b double mutants. Data are mean ± SD (n = 4). Asterisks indicate significant differences from control (Student’s t-test, * P < 0.05, ** P < 0. 01, *** P < 0.001).