Figure 4. BnPHT5;1bs functionally complement AtPHT5;1 in the Arabidopsis Atvpt1 mutant at higher Pi supplies.
(a) Phenotype of 4-week-old Col-0, vpt1 and BnPHT5;1b/vpt1 seedlings grown with various Pi concentrations. 6-day-old seedlings grown on 1/2 MS (Murashige and Skoog) medium (Murashige and Skoog, 1962) were transferred to ANS solution (Arabidopsis nutrient solution; Liu et al., 2016) supplemented with 13 μM, 130 μM, 1300 μM or 3,900 μM Pi for 3 weeks. Scale bars = 5 cm.(b) Fresh weight and (c) total P concentration of Col-0 (wild type), vpt1 and BnPHT5;1b/vpt1 lines in (a). Mean values \(\pm\)SD are shown (n = 4). Different lowercase letters above columns indicate a significant difference among different lines under each P treatment (P < 0.05 by Tukey’s test).