Figure 6. Disruption of BnPHT5;1b causes slower growth ofBrassica napus under P-sufficient and high P conditions.
(a) Phenotypic comparison of W10 and BnPHT5;1b DM lines under Pi-sufficient and high Pi conditions. 10-day-old seedlings grown in Hogland solution containg 100 μM Pi were transferred to 100 μM and 1,000 μM Pi, respectively for 7 days growth. (b) The fresh weights,(c) root-crown ratios and (d) Pi concentrations of seedlings shown in panel (a). (e) Percentage increase in Pi concentration in W10 and BnPHT5;1b DM plants between 100 μM and 1,000 μM Pi. Data are mean ± SD (n=4). W10, Westar 10; BnPHT5;1b DM #25 and BnPHT5;1b DM #31 , BnPHT5;1b double mutants. Asterisks indicate significant differences from control (Student’s t-test, * P < 0.05, ** P < 0. 01, *** P < 0.001).