Figure
5. Vacuolar Pi concentrations are significantly reduced in roots of theBnpht5;1b double mutant.
(a) BnPHT5;1b double mutants with different types of
mutation generated by the Crispr-cas9 system. (b, c) Pi
concentrations in the root vacuoles of Westar 10 and a BnPHT5;1bdouble mutant (BnPHT5;1b DM #25 ). Plants grown with 100 μM Pi
supply were transferred to 1,000 μM Pi for 2 hours before conducting
Nuclear Magnetic Resonance (NMR) spectroscopy analysis. Ref, methylene
diphosphonate (MDP). Data are mean ± SD (n=3). Asterisks indicate
significant differences from control (Student’s t-test, * P <
0.05).