Figure 6. Disruption of BnPHT5;1b causes slower growth ofBrassica napus under P-sufficient and high P conditions.
(a) Phenotypic comparison of W10 and BnPHT5;1b DM lines under
Pi-sufficient and high Pi conditions. 10-day-old seedlings grown in
Hogland solution containg 100 μM Pi were transferred to 100 μM and 1,000
μM Pi, respectively for 7 days growth. (b) The fresh weights,(c) root-crown ratios and (d) Pi concentrations of
seedlings shown in panel (a). (e) Percentage increase in Pi
concentration in W10 and BnPHT5;1b DM plants between 100 μM and
1,000 μM Pi. Data are mean ± SD (n=4). W10, Westar 10; BnPHT5;1b
DM #25 and BnPHT5;1b DM #31 , BnPHT5;1b double mutants.
Asterisks indicate significant differences from control (Student’s
t-test, * P < 0.05, ** P < 0. 01, *** P <
0.001).