3.1 nrITS and cpDNA dataset acquisition and assessment
Twelve ITS ribotypes were identified based on 16 variable nucleotide sites. The ITS1/2 length was 701 bp, nucleotide diversity (π) was 0.00390, the average number of nucleotide difference (K) was 2.681, and haplotype diversity (Hd) was 0.810 (Table 2). The geographical distribution of ribotypes showed that only a few ribotypes were shared across the species distribution range, whereas most of them were private to a single population (Table 1, Figure 1).
The two cpDNA sequences used contained 50 polymorphic sites, and yielded 19 chlorotypes. Nucleotide diversity (π) was 0.00860, average number of nucleotide difference (K) was 11.634, haplotype diversity (Hd) was 0.919 (Table 2). All chlorotypes were exclusive to a single or a group of neighbouring populations (Table 1, Figure 1). The total length of the combined chloroplast alignments was 1404 bp (730 bp and 674 bp in size for trn L-trn F and ycf 1b, respectively), a congruency test implied a high level of homogeneity of the two chloroplast sequences (P > 0.5) and a striking non-homogeneity between nrDNA and cpDNA (P < 0.5). Ribotype and chlorotype diversities (Hd) of the 10 sampled populations ranged from 0 to 0.586 and 0 to 0.711 respectively (Table 1).