2.3 Genetic diversity
The chromatograms from both directions of the ITS1/2 and cpDNA sequences were edited with the software BioEdit (Hall, 1999) for base confirmation and contiguous sequences editing. The sequences were manually aligned where necessary using MEGA v.6.5 (Kumar et al. , 2008). All sequences have been deposited in Genbank. The two non-coding cpDNA sequences were assembled as a single locus by SequenceMatrix v.1.7.8 (Vaidya et al., 2011), and a Partition Homogeneity Test (PHT) vs the ITS sequences was carried out with PAUP* v. 4.0a164 (Swofford, 2002). Since the test resulted in the non-homogeneity of both matrices, we analysed the geographic distribution of ribotypes/chlorotypes for nrITS and cpDNA sequences separately.
DNASP v. 6.12.01 (Rozas et al. , 2017) was used to compute the number of ribotype/chlorotype (h ), haplotype diversity (Hd) within populations, polymorphic sites (S), nucleotide diversity (π), average number of nucleotide difference (K).