Allicin Reduced the mRNA Expression of Chemokines and Antimicrobial Peptides (AMPs) both in IMQ-induced psoriatic lesions and IL-17A Stimulated HaCaT Cells
IL-17A is a potent stimulator of multiple immune-related proteins such as chemokines and AMPs, which lead to further inflammatory responses in keratinocytes of psoriasis lesions(Furueet al. , 2020). Excessive expression of AMPs in psoriatic lesions is involved in the pathogenesis of dermatoses via host inflammatory reactions, such as cathelicidin, β-defensins, and S100 proteins(Wang et al. , 2018). To investigate whether allicin could reduce the production of chemokines and AMPs by keratinocytes upon IL-17A stimulation, we measured the mRNA expression of CXCL8, CCL20, S100A8, and S100A9 by RT-qPCR. The data showed IL-17A remarkedly upregulated levels of CXCL8, CCL20, S100A8, and S100A9, which were notably reduced in the allicin-treated group (Figure 7A). Similarly, compared with the IMQ-group, allicin significantly decreased the mRNA expression levels of CXCL2, CXCL5, CCL20, S100A8, and S100A9 in psoriasis-like lesions (Figure 7B).