Cell Culture
The human immortalized epidermal keratinocyte cell line HaCaT was
purchased from the iCell Bioscience Inc (Shanghai, China, passages
20–22) and maintained in high-glucose Dulbecco’s Modified Eagle Medium
(DMEM, BIOIND, Israel) contained 10%
fetal bovine serum (FBS, BIOIND, Israel) and 1% penicillin-streptomycin
(PS, NCM Biotech, China) under standard conditions (37°C with 95%
humidity and 5% CO2). Cells were digested by 0.25%
trypsin-EDTA and terminated by DMEM containing 10% FBS.