Cell apoptosis assay
Annexin V-FITC Apoptosis Detection Kit (Beyotime, China) was used to quantitatively evaluate allicin-induced apoptosis. The supernatant was collected and mixed with HaCaT cells detached using EDTA-free trypsin. After centrifugation, HaCaT cells were resuspended in PBS. Then cells were incubated with 195μl binding buffer and incubated with 5μl Alexa Fluor 488 and 10μl PI for 15 min at room temperature. The proportion of cell apoptosis was measured by flow cytometry and analyzed with FlowJo V10.