Cell apoptosis assay
Annexin V-FITC Apoptosis Detection Kit (Beyotime, China) was used to
quantitatively evaluate allicin-induced apoptosis. The supernatant was
collected and mixed with HaCaT cells detached using EDTA-free trypsin.
After centrifugation, HaCaT cells were resuspended in PBS. Then cells
were incubated with 195μl binding buffer and incubated with 5μl Alexa
Fluor 488 and 10μl PI for 15 min at room temperature. The proportion of
cell apoptosis was measured by flow cytometry and analyzed with FlowJo
V10.