Toxicity Assay of Repeated Administration and Skin Irritation Test
New Zealand rabbits (both sexes, 14-16 weeks, 2.3-2.7 kg) were purchased from Qindao Kangda Biological Technology (Shandong, China), randomly assigned into four groups (n=10) contained control-group (blank solvent), allicin low-dose group (2 mg/g, measured with alliin), allicin middle-dose group (4 mg/g, measured with alliin), and allicin high-dose group (8 mg/g, measured with alliin). After four consecutive months of topical administration of allicin, body weight, organ weight, hematologic and clinical chemistry analysis were evaluated. Skin irritation included erythema and edema and was evaluated based on the above criteria. Stimulus Scores = Total Cumulative Stimulus Scores / Total Observation Times. Stimulation grade: without irritation (0-0.49), mild irritation (0.5-2.99), moderate irritation (3.0-5.99), severe irritation (6.0-8.00).