Fv/Fm during P.
syringae DC3000 infection.
Given the protection offered by MAMPs toFv/Fm levels during DC3000
infection, we next tested whether similar protection was also conferred
by plant derived Damage Associated Molecular Patterns (DAMPs). Using Pep
elicitors, Col-0 leaves were first pre-treated with Pep1, Pep2 or Pep3
(all at 1 µM) 16 h prior to DC3000 challenge.Fv/Fm dynamics over 24 h revealed
that Pep1 and 3 not Pep2 protected from DC3000Fv/Fm suppression (Figure 5A, B).
Critically, Pep1 and 3 pre-treatment failed to alter DC3000Fv/Fm infection dynamics in thepepR1-1x2-1 mutant (Yamaguchi et al. , 2010) (Figure 5C, D)
whereas flg22 pre-treated pepR1-1x2-1 leaves protected fromFv/Fm suppression as seen above
for Col-0 flg22 pre-treatment (Figure 5E, F). Interestingly, Pep1 and
Pep3 pre-treatment only provided partial protection againstFv/Fm suppression in DC3000
infected fls2 leaves and no protection in bkk1-1/bak1-5plants (Sup Figure 2a-d). These data indicate a degree of cross
protection of Fv/Fm between DAMPs
and MAMP priming of the plant.