Comparison of MiSeq barcodes with Sanger barcodes
Full-length barcodes were successfully recovered by both MiSeq and
Sanger barcoding approaches for 68 of 96 specimens subjected to both
methods. Full length Illumina barcodes were obtained from seven
specimens, and partial Illumina barcodes (one LCO-only and eight
BR-only) from a further nine specimens, that each failed to produce
Sanger barcodes; 15 of these 16 specimens were beetles. On the other
hand, only partial barcodes (one LCO-only and nine BR-only) were
recovered using the Illumina method from ten specimens from which
full-length Sanger barcodes were obtained. No barcodes were obtained
using either method from just two specimens (Ephutomorpha
bivulnerata , a wasp; and the single mite specimen).
Among 68 specimens from which both Sanger and Illumina barcodes were
recovered, pairwise sequence identities between these barcodes were from
75.5 % to 85.7 % for three specimens, 97.6 % to 98.6 % for six
specimens, 99.1 % to 99.9 % for 18 specimens, and 100 % for 40
specimens.