DNA quantity and quality
All samples were quantified with either the dsDNA high sensitivity or
broad range kits on the Qubit (Invitrogen, ThermoFisher). In the case of
the chinook salmon, a TaqMan qPCR specific assay was used (Shelton et
al., 2019) to quantify the amount of target DNA of each DNA extraction
using gBlocksTM fragments (synthetic double stranded
oligonucleotides from Integrated DNA Technologies Inc.) as standards
from 105 to 1 copies µl-1 and assess
the target to total ratio. The integrity of the DNA (DIN) was also
evaluated using the Genomic DNA ScreenTape and kit on a 2200 TapeStation
System (Agilent Technologies)