Table 3 Results of the sequencing process and assemblies from
three different enrichment methods and different tissue and species.Extraction (method): gDNA - total DNA extraction with phenol-chloroform;
mtDNA: extraction with phenol-chlroform from isolated mitochondria.
Enrich. method A: mitoenrichment; B: targeted mitosequencing; C: genome
skimming after whole genome sequencing. Underlined sample ID indicate
those that are available in GenBank with accessions ON005612-ON005619.
Figure 3. Maximum, mean and minimum coverage per treatment
(mitoenrichment and targeted mitosequencing), species and sample.Targeted mitosequencing average read depth is overall 10-fold the read
depth of the mitoenrichment approach but with more variability within
the mitogenome while the mitoenrichment coverage is more uniform. Sample
name coding is T: mitochondria selection with ThermoFisher Mitochondria
Isolation kit for cultured cells followed by phenol-chloroform DNA
extraction; PCI: direct genomic DNA phenol-chloroform extraction; L:
liver; H: heart (no notation for skeletal muscle). Note that run Ot-T-6
was performed on a flongle flow cell and run Ot-T-H24 performed on a
flongle flow cell that died before two hours of sequencing is not
included.