Comparing mitonerichment to targeted mitosequencing
The total number of reads was greater on the mitoenrichment method,
however the proportion and number of sequences on target was greater for
the targeted mitosequencing method (Table 3, S3, Fig. 2). On the other
hand, the read depth was more evenly distributed across the mitogenome
with the mitoenrichment method despite the average read depth being
greater for the targeted mitosequencing approach. The use of liver
increases the ratio of sequences on target for the mitoenrichmnent
approach while also increasing the off-target number of sequences, which
turns into a greater use of the flow cell. On the contrary, the heart
does not perform better than skeletal muscle (Table 3, Fig.3). The more
degraded the sample (low DIN) the lower the coverage and the shorter the
reads were. Further enrichment was achieved for samples extracted from
isolated mitochondria and treated with the Cas9 targeted sequencing
method (Fig 3)
Figure 2. Overall representation of all runs and species for the
proportion and number of sequences on-target and mean coverage by
enrichment method. The targeted mitosequencing method has a greater
proportion of sequences on target and deeper coverage overall.