Table 3 Results of the sequencing process and assemblies from three different enrichment methods and different tissue and species.Extraction (method): gDNA - total DNA extraction with phenol-chloroform; mtDNA: extraction with phenol-chlroform from isolated mitochondria. Enrich. method A: mitoenrichment; B: targeted mitosequencing; C: genome skimming after whole genome sequencing. Underlined sample ID indicate those that are available in GenBank with accessions ON005612-ON005619.
Figure 3. Maximum, mean and minimum coverage per treatment (mitoenrichment and targeted mitosequencing), species and sample.Targeted mitosequencing average read depth is overall 10-fold the read depth of the mitoenrichment approach but with more variability within the mitogenome while the mitoenrichment coverage is more uniform. Sample name coding is T: mitochondria selection with ThermoFisher Mitochondria Isolation kit for cultured cells followed by phenol-chloroform DNA extraction; PCI: direct genomic DNA phenol-chloroform extraction; L: liver; H: heart (no notation for skeletal muscle). Note that run Ot-T-6 was performed on a flongle flow cell and run Ot-T-H24 performed on a flongle flow cell that died before two hours of sequencing is not included.