2.6 Determination of emulsion droplet size
The droplet size of the emulsions was determined by analyzing the images
taken with the confocal microscope. It was essential to select multiple
images of each sample so that the number of water droplets quantified
was between 3,000 – 18,000. The image analysis methodology developed in
Romero-Peña et al. (2020) was used to quantify the water droplet size
using ImageJ software (v1.5 2i – Fiji project). The image analysis data
(area of each droplet) was exported to an Excel spreadsheet (Microsoft
Office 365), and further calculation was done to obtain the
volume-weighted means droplet diameter (d 4,3)
using Eq. 2, where d is the diameter of each droplet obtained
from the image analysis and n is the number of droplets of a
particular size.
\(d_{4,3}=\frac{\sum_{1}^{n}d^{4}}{\sum_{1}^{n}d^{3}}\) Eq. 2