2.6 Determination of emulsion droplet size
The droplet size of the emulsions was determined by analyzing the images taken with the confocal microscope. It was essential to select multiple images of each sample so that the number of water droplets quantified was between 3,000 – 18,000. The image analysis methodology developed in Romero-Peña et al. (2020) was used to quantify the water droplet size using ImageJ software (v1.5 2i – Fiji project). The image analysis data (area of each droplet) was exported to an Excel spreadsheet (Microsoft Office 365), and further calculation was done to obtain the volume-weighted means droplet diameter (d 4,3) using Eq. 2, where d is the diameter of each droplet obtained from the image analysis and n is the number of droplets of a particular size.
\(d_{4,3}=\frac{\sum_{1}^{n}d^{4}}{\sum_{1}^{n}d^{3}}\) Eq. 2