2.5.2 Confocal microscopy
Emulsion microstructure was also captured with a confocal microscope
(Nikon C2, Nikon Inc., Mississauga, ON, Canada). One drop of 0.1 wt%
Nile red solution in mineral oil was mixed with nine drops of fresh
emulsion, and a drop of this mixture was placed over a glass depression
microscope slide covered with a coverslip and placed under the
microscope. The sample was excited using a 543 nm laser, and the
emission spectra were collected from 573 to 613 nm using a 40x and 60x
objectives lens.