2.5.2 Confocal microscopy
Emulsion microstructure was also captured with a confocal microscope (Nikon C2, Nikon Inc., Mississauga, ON, Canada). One drop of 0.1 wt% Nile red solution in mineral oil was mixed with nine drops of fresh emulsion, and a drop of this mixture was placed over a glass depression microscope slide covered with a coverslip and placed under the microscope. The sample was excited using a 543 nm laser, and the emission spectra were collected from 573 to 613 nm using a 40x and 60x objectives lens.