2.5 Statistical analysis
The statistical analyses were conducted with IBM SPSS Statistics 26.0. A comparison of antibodies and T-cell reaction between groups were analyzed using the Mann-Whitney U-test and Paired Wilcoxon Signed-Rank Test. The difference in positive rate was performed by the Chi-square test. The figures were done with GraphPad Prism 8.0.2. The statistical significance of these results was defined as * p < 0.05, ** p < 0.01, and *** p < 0.001.