Genes Primer sequence (5′-3′) Annealing temperature (°C) Annealing temperature (°C) Product size (bp) Product size (bp)
GHR F: ACACGTCCATTTGGATCCCC F: ACACGTCCATTTGGATCCCC 58 58 183
R: GCTCCCAGTTGACCATGACA R: GCTCCCAGTTGACCATGACA
IGF-1 F: CTGAGAGAGCGGAACCATCC F: CTGAGAGAGCGGAACCATCC 58 58 194
R: CAGATAATGAGCCGCCTGGT R: CAGATAATGAGCCGCCTGGT
IGF-1R( Jia, Yudong, et al.,2018) F: CCTGATGTCACAGTGGGTGT F: CCTGATGTCACAGTGGGTGT 58 58 82
R: GTCTTCCCACTCACTTTGCTG R: GTCTTCCCACTCACTTTGCTG
Ef1α (Wu, Lele, et al.,2021) F: CTGGGTGCTGGACAAACTG F: CTGGGTGCTGGACAAACTG 58 58 220
R: GTTCTTGGAAATACCTGCCTC R: GTTCTTGGAAATACCTGCCTC