Figure 3. Genome-wide association study of low-temperature
resistance in a panel of B. napus accessions.
(A) Manhattan plots and Q-Q plots for genome-wide association
study of low temperature resistance phenotype measured in Year 2017
(top) and Year 2018 (bottom), respectively. (B) qRT-PCR
analysis of the abundance of three candidate miRNAs under 22 °C and 4 °C
condition for 0, 10, 20 and 30 days, respectively. Error bar represents
mean ± SE; n = 3. U6 was used as the endogenous reference
gene. * indicates P < 0.05; ** indicates P< 0.01; Student’s t-test. (C ) Haplotypes of candidate
SNPs within MIR1885 promoter for low-temperature resistance inB. napus population. LTR represents low-temperature element
(CCGAAA). Five variants, including four SNPs and the one-bp deletion,
which were significantly associated with low-temperature resistance,
were marked with dark bold vertical lines. The five variants located in
the 200-bp genomic region around LTR were used for analysis. ‘Hap.’
denotes the haplotypes of MIR1885 ; ‘Number’ denotes the number of
lines belonging to each haplotype group. ‘Degree’ denotes the average
grade of low-temperature resistance.