Figure S7. Freezing sensitivity of miR1885 over-expressing transgenic plants.
(A) Phenotypes of transgenic lines grown at 22 °C and −10 °C. Plants were grown at 22 °C for 6 weeks (upper panel), and then transferred to −10 °C for 2.5 h (middle panel), and then allowed to recover at 22 °C for 7 days. (B) Real-time PCR showing the expression pattern of miR1885 under freezing treatment. U6 was used as endogenous reference gene. Error bar represents mean ± SD;n = 3. (C) Survival rates of transgenic lines and wild-type plants after freezing treatment (−10 °C in a growth chamber for 2.5 h, n = 15–20 plants). * indicates P < 0.05; ** indicates P < 0.01 (Student’s t -test).(D) Proline content in transgenic plants and wild type after freezing treatment. Error bar represents mean ± SD. * indicatesP < 0.05, significant difference from plants subjected to −10 °C before freezing treatment (0 h). † indicates P< 0.05, significant difference from plants subjected to −10 °C for 2.5 h (Student’s t -test).