Plasmids and strains construction
All plasmids constructed in the current study were conducted by using
Guanin/Thymine standard (Ma et al., 2019). Constructed plasmids were
verified by Sanger sequencing (service provider: Bio Basic Asia Pacific
Pte Ltd, Singapore). Each of the constructed plasmids was introduced
into E. coli MG1655_DE3 (ΔrecA, ΔendA, DE3) via
standard electroporation protocol (Ma et al., 2019). Successfully
constructed strains were isolated on Luria Bertani (LB) agar (BD, DIFCO)
plate containing proper antibiotics. All plasmids and strains used in
this study are summarized in Table 1 .
Table 1 . Plasmids and strains used in this study.