Plasmids and strains construction
All plasmids constructed in the current study were conducted by using Guanin/Thymine standard (Ma et al., 2019). Constructed plasmids were verified by Sanger sequencing (service provider: Bio Basic Asia Pacific Pte Ltd, Singapore). Each of the constructed plasmids was introduced into E. coli MG1655_DE3 (ΔrecA, ΔendA, DE3) via standard electroporation protocol (Ma et al., 2019). Successfully constructed strains were isolated on Luria Bertani (LB) agar (BD, DIFCO) plate containing proper antibiotics. All plasmids and strains used in this study are summarized in Table 1 .
Table 1 . Plasmids and strains used in this study.