2.4 Scanning Electron Microscopy
Scanning electron microscopy (SEM) was used to study cells immobilized
on the fibrous matrix. Small pieces of fibrous matrices with cells cut
from various locations in the FBB were examined at 20 kV with FEI Quanta
200 SEM. Samples were fixed in 2.5% glutaraldehyde solution at 4 °C
overnight, dehydrated with ethanol progressively from 20% to 100% at
20% increment for 30 min at each step, dried with hexamethyldisilazane
(HMDS), and spotter-coated with gold particles under argon gas. The
samples were scanned, photographed, and analyzed visually for cell
morphology.