Phylogenetic analysis
Phylogenetic relatedness of ERF-VII proteins was analysed using MEGA 7
(Kumar et al. , 2016). The full length of the coding regions
(amino acid sequences) was aligned using Clustal Omega (Sievers et
al. , 2014). A phylogenetic tree was constructed by the
Neighbour-Joining method with Poisson correction and pairwise deletion
of gaps. The reliability of the output phylogeny was estimated through
bootstrap analysis with 1000 replicates.