SHORT LEGENDS FOR SUPPORTING INFORMATION
Supplemental Figure 1: Localization of the specific region
sequence targeted by the RNAi.
Supplemental Figure 2: In silico analysis ofMtERF-VII gene expression in M. truncatula .
Supplemental Figure 3: Validation of effectiveness and
specificity of the MtERF74/75 RNAi constructs.
Supplemental Figure 4: Root growth and secondary roots
phenotypes in composite knock-down roots transformed with theMtERF74/75 :RNAi vector or with GUS vector.
Supplemental Figure 5: Effect of MtERF74/75 RNAi on transcripts
level of some hypoxia-responsive genes in M. truncatula root
submitted to 24 hours of hypoxia stress in hypoxic chamber.
Supplemental Figure 6: Identification of Tnt1 mutant
line NF1722 for MtERF74 genes.
Supplemental Figure 7: Comparison of the level of expression of
the ERFVII:: MA and MC constructs.
Supplemental Figure 8: Mass spectrometric analyses on CR20
peptide treated to identify S-nitrosylation post-translational
modification.
Supplemental Table 1: Primer sequences for cloning and
quantitative RT-PCR analysis.
Supplemental Table 2: All Plasmids used in this study.
Supplemental Table 3: Nomenclature of M. truncatulaERF-VII
Supplemental Table 4: Transcriptional profiles of M.
truncatula genes in 3 weeks old nodules compared to the root.