Luciferase activity assay
Arabidopsis thaliana protoplasts were prepared according to the
method described in Yoo et al. (2007) and transfected using 5 μg plasmid
DNA of p2G-MC/MA80_75:LUC and p2GRenLuc (Licausi et al. , 2011).
The luciferase activity assays were performed directly on protoplast
suspension with Dual-Glo® Luciferase Assay System (Promega). The
luminescent signal was measured on a Xenius XL luminometer (SAFAS,
Monaco) and values normalized against the Renilla luminescence. The data
are represented as the ratio of firefly to Renilla luciferase
activity (Fluc/Rluc).