Figure legend
Figure 1 . Functional and structural characterization of recombinant IgE antibodies. Chimeric human/rabbit IgE antibodies were generated by fusion of rabbit anti-HRP scFvs and human IgE constant domains. The recombinant IgEs (rIgE) recognized CCD-containing extracts from bee and yellow jacket venom and rape pollen (1), the CCD-carrying glycoproteins HRP, HSA-MUXF and nPhl p 4 (2), but not the recombinant wasp allergens rVes v 1 and rVes v 5 lacking CCDs (3). The rIgEs caused mediator release from effector cells (SX38) (4). Crystal structures of a complex between the Fab fragment and the disaccharide FucαGlcNAcα1-OMe (Fuc-mNAG) were generated (5) which allowed structural characterization of the interaction between the CCDs and antibodies.