IFN-γ production and LDH release evaluation
Human Lactate dehydrogenase (LDH) and Interferon-gamma (IFN-γ) ELISA
Kits were used to test LDH release from the KG1 cell line, and IFN-γ
production by NK cells, following the manufacturer’s procedure. Briefly,
100 μL per well of standard solutions or samples was divided into
aliquots in duplicate into a precoated 96-well plate. After discarding
the plate content, 100 μL/well of biotinylated anti-human LDH and IFN-γ
antibodies were added to each well. After rinsing three times with PBS,
a prepared ABC working solution (100 μL/well) was added to each well.
Finally, after washing with PBS-, a prepared stop
solution of 100 μL/well was added to each well, and the plate was read
at 450 nm in a microplate reader.