Expression of granzyme A and B in different formulations of NK activators
Granzymes are derived from serine proteases and expressed in cytotoxic T cells and NK cells 15. GZMA and GZMB are carried out and stored in the particular granules of resting NK cells and released after activation 16. Therefore, we assessed the expression of GZMA and GZMB in different groups of activated NK cells from seven patient with AML. The results indicated that IL-15 + Hsp70 + PD-1 blocker group upregulated the expression of both GZMA and GZMB (p<0.01, Fig. 4E and 4F ). Moreover, the lytic granules of NK cells contain the pore-forming protein perforin that, after the formation of immune synapses, creates a pore in the membrane of the target cell; finally, granzymes may enter the target cell cytoplasm and cleave several substrates, leading to apoptosis via the intrinsic pathway 17. our result also showed that expression of PRF-1 in NK cells exposed to IL-15 + Hsp70 + PD-1 blocker was increased compared to all other groups (Fig. 4G).