Cell culture and Reagents 
MNCs were harvested from Peripheral blood using Ficoll-Hypaque density gradient centrifugation. The Ficoll-Paque centrifugation is done as per the manufacturer’s protocol. The KG-1 cell line was purchased from Royan Institute Cell Bank (Tehran, Iran). KG-1 cells were cultured in RPMI-1640 containing 10% FBS (GIBCO, Cat. No: 26140-079), 3 mM L-glutamine (GIBCO, Cat. No: 25030-024), 1% Pen/Strep antibiotics (GIBCO, Cat. No: 15070-063), and a humidified atmosphere of 5% CO2 at 37 °C.