Immunophenotyping of cells
Peripheral Blood Mononuclear Cells (PBMCs) derived from Patients with AML, were stained with FITC anti-human CD335 (NKP46; Cat. No: 331922), PE anti-human CD159a (NKG2A; Cat. No: 142803), PerCp/Cyanine5.5 anti-human CD337 (NKP30; Cat.No:325216), PE anti-human CD314 (NKG2D; Cat. No: 320806), and BD Simulates CD3/CD16+CD56 (Cat. No: 342403) to evaluate the abundance, phenotype and function of NK cells by BD FACS Calibur and analyzed in FlowJo software Ver.10.6.1. All fluorescence-labeled antibodies were acquired from Biolegend (USA).