Expression of granzyme A and B in different formulations of NK
activators
Granzymes are derived from serine proteases and expressed in cytotoxic T
cells and NK cells 15. GZMA and GZMB are carried out
and stored in the particular granules of resting NK cells and released
after activation 16. Therefore, we assessed the
expression of GZMA and GZMB in different groups of activated NK cells
from seven patient with AML. The results indicated that IL-15 + Hsp70 +
PD-1 blocker group upregulated the expression of both GZMA and GZMB
(p<0.01, Fig. 4E and 4F ). Moreover, the lytic
granules of NK cells contain the pore-forming protein perforin that,
after the formation of immune synapses, creates a pore in the membrane
of the target cell; finally, granzymes may enter the target cell
cytoplasm and cleave several substrates, leading to apoptosis via the
intrinsic pathway 17. our result also showed that
expression of PRF-1 in NK cells exposed to IL-15 + Hsp70 + PD-1 blocker
was increased compared to all other groups (Fig. 4G).