IFN-γ production and LDH release evaluation
Human Lactate dehydrogenase (LDH) and Interferon-gamma (IFN-γ) ELISA Kits were used to test LDH release from the KG1 cell line, and IFN-γ production by NK cells, following the manufacturer’s procedure. Briefly, 100 μL per well of standard solutions or samples was divided into aliquots in duplicate into a precoated 96-well plate. After discarding the plate content, 100 μL/well of biotinylated anti-human LDH and IFN-γ antibodies were added to each well. After rinsing three times with PBS, a prepared ABC working solution (100 μL/well) was added to each well. Finally, after washing with PBS-, a prepared stop solution of 100 μL/well was added to each well, and the plate was read at 450 nm in a microplate reader.