Cell culture and Reagents
MNCs were harvested from Peripheral blood using Ficoll-Hypaque density
gradient centrifugation. The Ficoll-Paque centrifugation is done as per
the manufacturer’s protocol. The KG-1 cell line was purchased from Royan
Institute Cell Bank (Tehran, Iran). KG-1 cells were cultured in
RPMI-1640 containing 10% FBS (GIBCO, Cat. No: 26140-079), 3 mM
L-glutamine (GIBCO, Cat. No: 25030-024), 1% Pen/Strep antibiotics
(GIBCO, Cat. No: 15070-063), and a humidified atmosphere of 5%
CO2 at 37 °C.