Treatment with antihistamines
Passively sensitized PCIS were incubated with diphenhydramine
hydrochloride (10µg/ml), cetirizine dihydrochloride (1µg/ml) and
rupatadine (1µg/ml) (MilliporeSigma, Darmstadt, Germany) for 10min prior
to stimulation with histamine (10µg/ml) and filmed for 10min
post-stimulation. Concentrations were chosen based on published reports
and dose-response experiments.32–35