Treatment with antihistamines
Passively sensitized PCIS were incubated with diphenhydramine hydrochloride (10µg/ml), cetirizine dihydrochloride (1µg/ml) and rupatadine (1µg/ml) (MilliporeSigma, Darmstadt, Germany) for 10min prior to stimulation with histamine (10µg/ml) and filmed for 10min post-stimulation. Concentrations were chosen based on published reports and dose-response experiments.32–35