3.2 Whole leaf analysis
OPEN Leaf captures whole images of plant to track the overall
development of the plant dynamically through time. The two most
important characteristics to observe in plant development, specifically
for nutrient uptake, is the change in area and color14(Figure 2). These approaches provide many valuable insights into plant
development and the characterization of phenotypes. However, dynamic
detail that occur on leaf level are often overlooked by only observing
plant as a whole. For example, iron deficiency displays itself primary
in younger leaves, and therefore, when observing the whole plant, area
changes very little since older leaves are consistent in growth and
color is minimally impacted21. Ergo, it is important
to observe growth of leaves independently of the entire plant.