3.2 Whole leaf analysis
OPEN Leaf captures whole images of plant to track the overall development of the plant dynamically through time. The two most important characteristics to observe in plant development, specifically for nutrient uptake, is the change in area and color14(Figure 2). These approaches provide many valuable insights into plant development and the characterization of phenotypes. However, dynamic detail that occur on leaf level are often overlooked by only observing plant as a whole. For example, iron deficiency displays itself primary in younger leaves, and therefore, when observing the whole plant, area changes very little since older leaves are consistent in growth and color is minimally impacted21. Ergo, it is important to observe growth of leaves independently of the entire plant.