Figure 3. Leaf specific analysis provide new insights to nutrient deficiency phenotypes by observing the changes of area and color one leaf at a time
CONCLUSION
OPEN Leaf addresses many of the current limitations and inconveniences of HTP systems in predictive biology. OPEN Leaf is open-source and accessible, characterizes abiotic stress responses within hydroponics, integrated data management throughCyVerse , and automated image processing. Many high-resolution HTP systems are commercial and outside of budgets for most labs. OPEN Leaf is $1000 and entirely open source. OPEN Leaf characterizes abiotic stress responses at a whole plant and leaf-specific to aid in understanding the genetically complex systems involved in stress response. Many other open-source HTP systems have no method for data management and processing. OPEN Leaf utilizes CyVerse to both manage and process data; allowing labs across the globe to participate in collaborative research. Future projects in the OPEN series includes increasing throughput of OPEN Leaf and a root phenotyper with similar OPEN principles.