Cell flow cytometry.
For testing the efficiency of d2eGFP targeting, flow cytometry was used.
HEK293T cells were seeded at 1.8×105 per well in a
24-well plate 18-24 h before transfection, and 1 μg remodeled plasmid
PCAG::Cas13d-P2A-d2eGFP-PU6::CasRfx-crRNA was transfected by
lipofectamine 8000. FACS was performed at 48 hours post-transfection.
All transfection experiments were performed in a way of biological
triplicates.