Cell lines, culture, and transfection
HEK293T cells were cultured in complete DMEM high glucose medium
(Hyclone) supplemented with 10% heat-inactivated fetal bovine serum and
maintained in a humidified atmosphere of 5% CO2 at 37
°C. Cells were transfected with plasmid by Lipofectamine™ 3000
Transfection Reagent (Thermo Fisher, USA) or Lipo8000™ Transfection
Reagent (Beyotime, China).