FIGURE
1 A cell-free framework for enzyme homolog prototyping and pathway
optimizing by combining cell-free protein synthesis (CFPS) with split
GFP
assay.
(a)
Biosynthetic pathways are built in a modular assembly manner. (b)
Schematic representation of the strategy for the normalized screening of
productive homologs. (c) Schematic representation of the workflow for
building
optimized biosynthetic pathway in purified enzyme systems.