Figures
Figure 1. A schematic overview of the common garden experiment.
The Japanese populations were collected on September the
14th (Fuitatsuiwa; fut ) and
15th (Akkeshi; akk ) and arrived in the climate
room on September the 17th. The populations from
Germany were collected and brought to the climate room on September the
20th (Nordstrand; nor ) and September the
21st (Kiel; dhd ). The antibiotic treatment
started September the 26th and lasted 24 hours. Algae
were subsequently separated in two parts into different tanks at 15 °C
and 22 °C. These tanks also received the inoculum: a combination of
living algal branches from all population in a 50 mL tube with openings,
to function as a source of microbes. Epibiota were sampled in the field
(tf ), before the disturbance treatment
(t0) and 1,2 4 and 12 weeks following the disturbance
(t1 , t2 ,
t4 , t12 , respectively).
Figure 2. Marginal means of host performance traits recorded at
the end of the experiment (t12 ) with 95%
confidence intervals. The relative growth rate (RGR, A), measured as
percentage wet weight increase per day between temperatures and between
ranges (B). The probability of observing brittleness of the algal
thallus between temperatures (C) and between ranges (D). RGR graphs
include the partial residuals and for thallus brittleness the raw binary
data were plotted .
Figure 3. Rarefied OTU richness (to 1132 reads, panels A-E),
evenness in terms of logit transformed PIE (F-J) and within-population
beta diversity measured as Bray-Curtis (K-O) and Euclidean distances
(P-T) with 95% confidence limits and partial residuals. Marginal means
in the field (tfield ), before holobiont
disturbance (t0 ) and 7 days after holobiont
disturbance at 15 °C (t1 , A, F, K, P).
Post-disturbance responses over time at 15 °C (B, G, L, Q) and 22 °C (C,
H, M, R), by temperature (D, I, N, S) and by range within temperature
(E, J, O, T).
Figure 4. Non-metric dimensional scaling (nMDS) based on the
rescaled residuals from mGLMs including the LSD as offset to correct for
the effect of sequencing depth using Bray-Curtis (A-D) and Euclidean
distances (E-H). . Panels A and B include samples from pre- and
post-disturbance time points (tf ,
t0 , t1 ,
t2 , t4 and
t12 ).. Blue and red arrows draw the
post-disturbance trajectories of the 15 and 22 °C temperature groups in
time. The other panels show nMDS plots using rescaled residuals from
mGLMs including all terms, except the variable of interest; time (B, F),
temperature (C, G) and range (D, H). The 95% confidence regions of the
group centroids are shown as ellipses. Note that the smaller panels
(B-D, F-H) are zoomed in and some datapoints are outside the frame
limits.