2.9 Manual annotation of diapause genes
To annotate the genes involved to diapause, we used the protein sequence
of DH-PBAN, PTTH, , CRY 1, CRY 2, PER and TIM from Uniport
(https://www.uniprot.org/help/uniprotkb)
and NCBI (https://www.ncbi.nlm.nih.gov/) as queries to blast against the
protein sequence of C. japonica and other 9 insects by BLASTP
v2.8.1 (evalue: 1e-5). The prediction sequences from BLASTP were checked
by Hmmsearch v3.3.1 based on domains loaded from Pfam
(http://pfam.xfam.org) (DH: PF05874,
CRY 1&, CRY 2: PF03441, PER: PF12114, TIM: PF04821) (Potter et
al. , 2018).