2.2 Preparation of S1-SF-10
The procedure used for the preparation of SSF was described in detail
previously.14, 17 Briefly, SSF was prepared by mixing
DPPC, PG and PA, and K6L16 peptide at a weight ratio of 75:25:10:2. SSF
was mixed with S1 at a SSF phospholipid:S1 protein ratio of 10:1 at
42°C, and the S1-SSF complex was prepared by lyophilization and stored
at -20°C until use. To prepare 0.1% CVP solution, CVP powder
(Carbopol®971, Lubrizol, Cleveland, OH) was dissolved
in saline (pH 7.0-7.5) by stirring followed by homogenizer and then
stored at 4°C until use. Before administration, the lyophilized S1-SSF
was dissolved in 0.1% CVP solution.