Ultrasonographic anomalies were divided in three groups: 1 (fetuses with one ultrasonographic anomaly), 2 (fetuses with two ultrasonographic anomalies), and ≥ 3 (fetuses with more than two ultrasonographic anomalies). For group 1, ultrasonographic anomalies were further divided into 12 isolated anomalies. Each subgroup was compared with fetuses with no identifiable anomalies and maternal age ≥ 35 years. pCNV: pathogenic/likely pathogenic copy number variations. OR: odds ratio; CI: confidence interval. Binomial test with bonferroni correction was to compare yields between groups. Bold values denote statistically significant at the P value < 0.05 level.