3.11 Identification and analysis of differentially expressed genes after T. controversa and T. laevis infection
Gene expression comparison were performed among T. controversa ,T. laevis and their control samples at five different anther stages. For T. controversa , at <150μm, 3293 DEGs were identified, of which 2644 were up-regulated and 649 were down-regulated. For 150~400 μm anther length libraries, 2916 DEGs were identified, of which 1837 were up-regulated and 1079 were down-regulated. Similarly, for 400~700 μm, 3730 DEGs were generated, of which 1126 were up-regulated and 2604 were down-regulated. For 700~1100 μm, 1118 DEGs were identified, of which 767 were up-regulated and 351 were down-regulated. While for 1100~1300 μm, 2217 DEGs were identified, of which 1255 were up-regulated and 962 were down-regulated (Figure8A ). For T. laevis , at <150μm, 18,036 DEGs were identified, of which 10,445 were up-regulated and 7591 were down-regulated. In 150~400 μm anther length, whenT. laevis and control libraries were compared, 15,505 DEGs were generated with 5994 up-regulated and 9511 were down-regulated. Similarly, at 400~700 μm anther length, 4337 DEGs were identified, of which 1116 were up-regulated and 3221 were down-regulated. In 700~1100 μm anther length, 7909 DEGs were identified, of which 3246 were up-regulated and 4663 were down-regulated, while for 1100~1300 μm length, 15,261 DEGs were identified, of which 7121 were up-regulated and 8140 were down-regulated (Figure 8B ).