2.3 Molecular detection of T. laevis and T. controversa in inoculated wheat anther
DNA was extracted from wheat leaves using the cetyl trimethylammonium bromide (CTAB) method. The sequence characterized amplified region (SCAR) markers for T. laevis (Yao et al., 2019) and T. controversa (Gao et al., 2014) were used as previously described. Successful infection was indicated by positive amplification of the 660-bp (T. laevis ) or 372-bp (T. controversa ) specific band.