3.16 Validation of RNA-Seq data
Total RNA at five developmental stages during both pathogens infection provided templates for qRT-PCR validation. For T. laevis , we randomly selected 11 DEGs to validate our RNA-Seq results. The qRT-PCR data for these genes were consistent with the RNA-Seq results from the five anther lengths, which indicated a high degree of reproducibility between transcript abundances assayed using RNA-Seq and the expression profiles revealed by qRT-PCR data (Figure S6A ). For T. controversa , 8 DEGs of different anther lengths were randomly selected for validation. The RNA-Seq and qRT-PCR analysis results showed high degree of reproducibility (Figure S6B ).