4.5 TaEC contribute to wheat resistance to bunt pathogens
based on VIGS
To characterize in more detail the role of TaEC during wheat
response to bunt pathogens, BSMV-VIGS, which has been applied to
analysis gene function in wheat and barley, was used (Gao et al., 2013).
This is the first time to silence TaEC through BSMV-based VIGS in
wheat spikes against bunt pathogens. VIGS was done against Apple
latent spherical virus (CMV) and Cucumber mosaic virus (ALSV) in
soybean at different stages of growth development (Nagamatsu et al.,
2007; Yamagishi & Yoshikawa, 2009), inoculation of recombinant virus on
different parts of the plant. We first targeted the TaEC genes in
wheat. These results confirmed that VIGS successfully established in
wheat crops against T. controversa and T. laevisinfection. Additionally, provide a genome wide gene expression profile
for wheat crops infected by T. laevis and T. controversaand may help to explore the regulatory mechanisms governing the response
of wheat crops to these pathogens. According to our knowledge, it is the
first study to build up the timeline of wheat anther and reveal the
effects of both pathogens on wheat anther development and transcriptome
alterations by confocal analysis and RNA-seq, and additionally, firstly
establishment of VIGS to explore the gene function after infection of
two bunt pathogens.