3.7 Cell differentiation and expansion in T. laevisinfected anthers
Cell numbers (Figure5A-D ) and sizes (Figure 5E-G ), cell volume (Figure5H ) and shapes (y/x) (Figure 5I ) in the T.
laevis infected anthers were showed. Specifically, In Figure5A, Table 3 , after T. laevis infection, cell
counts of EPI, EN, SPL, ML, TA, PMC, MP and pollen grain (PG) along
X-axis changed during anther development, and significantly increased
response was noted in the ML of 700-1100 µm anthers. For EN, cell counts
were increased in 250-400 µm, and almost keep the same between 700-2300
µm. Additionally, ML cells had increased cell counts during 400-800 µm
anthers ( Figure 5B, Table 3). The total cells
per locule (X*Y) of EPI cells increase from 200-2300 µm, while in EN
cells increased much more in 200-400 µm anthers. In the case of ML and
TA, total cells per locule increased in 400-1100 µm and 700-1200 µm
respectively (Figure 5C, Table 3) . The percentage of
locule cell count of EPI cells statistically increased from 200-700 µm
infection. In contrast, the percent of EN cells significantly decreased
in 200-700 µm anther. However, the percentage of locule cell count of MP
and PG almost keep the same ( Figure 5D, Table3) . Results demonstrate that there was no significant change in
the cell width (X-axis) of TA, while for EN, which increased
from200-2300 µm anthers (Figure 5E, Table 4). The cell
length (Y-axis) significantly increased only in EPI cells during anther
development in 1100-1300 µm anthers (Figure 5F, Table4 ). For cell depth (Z-axis), the infected anthers did not show
much change in EPI, EN, SPL, ML, TA, PMC, MP cells in the developmental
period (Figure 5G, Table 4 ). The volume (X*Y*Z) of EPI
and EN cells increased from 150-2300 µm anthers. (Figure 5H,Table 4 ). Likewise, Y/X of EN cells decreased during early
anther development. The Y/X of SPL, ML, MP, PG cells almost keep the
same during anther development (Figure 5I, Table 4 ).
The percentage of locule volume of EPI, EN cells significantly decreased
in 150-400 µm and then increase (Figure 5J, Table 4) .
All above changes in the anther cell types suggested that T.
laevis infects the anther structure and create morphological changes.