3.16 Validation of RNA-Seq data
Total RNA at five developmental stages during both pathogens infection
provided templates for qRT-PCR validation. For T. laevis , we
randomly selected 11 DEGs to validate our RNA-Seq results. The qRT-PCR
data for these genes were consistent with the RNA-Seq results from the
five anther lengths, which indicated a high degree of reproducibility
between transcript abundances assayed using RNA-Seq and the expression
profiles revealed by qRT-PCR data (Figure S6A ). For T.
controversa , 8 DEGs of different anther lengths were randomly selected
for validation. The RNA-Seq and qRT-PCR analysis results showed high
degree of reproducibility (Figure S6B ).