2.9 Validation of transcript data
The first-strand cDNA was synthesized by using 1.5 µg purified RNA by
using Trans Script One-Step gDNA Removal and cDNA Synthesis SuperMix Kit
(TransGen Biotech, Beijing, China) and stored at -20 ˚C for further use.
PerfectStart Green qPCR SuperMix (TransGen Biotech, Beijing, China) kit
was used for qRT-PCR reactions. For validation of transcripts data of
significant DEGs, 11 genes were selected from both pathogen infected and
control samples for validation analysis.