4.5 TaEC contribute to wheat resistance to bunt pathogens based on VIGS
To characterize in more detail the role of TaEC during wheat response to bunt pathogens, BSMV-VIGS, which has been applied to analysis gene function in wheat and barley, was used (Gao et al., 2013). This is the first time to silence TaEC through BSMV-based VIGS in wheat spikes against bunt pathogens. VIGS was done against Apple latent spherical virus (CMV) and Cucumber mosaic virus (ALSV) in soybean at different stages of growth development (Nagamatsu et al., 2007; Yamagishi & Yoshikawa, 2009), inoculation of recombinant virus on different parts of the plant. We first targeted the TaEC genes in wheat. These results confirmed that VIGS successfully established in wheat crops against T. controversa and T. laevisinfection. Additionally, provide a genome wide gene expression profile for wheat crops infected by T. laevis and T. controversaand may help to explore the regulatory mechanisms governing the response of wheat crops to these pathogens. According to our knowledge, it is the first study to build up the timeline of wheat anther and reveal the effects of both pathogens on wheat anther development and transcriptome alterations by confocal analysis and RNA-seq, and additionally, firstly establishment of VIGS to explore the gene function after infection of two bunt pathogens.