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FIGURE 1: A) Cytopictograph from fine needle aspirate of digit 3: The hyphae are irregularly septate, exhibited acute and perpendicular branching and rarely exhibited large, round, bulbous ends that contained fine green to black pigmentation. Wright Giemsa, 50x
B) Cytopictograph from pericardial effusion. Fungal hyphae exhibited predominantly elongate septae with fewer short, cuboidal to rounded, regular septation that were present centrally.; Wright Giemsa, 100x.
FIGURE 2: A) Digits 3 and 4 of the right forelimb showing an open lesion and swelling of both digits. Localized Curvularia infection was suspected to have been acquired traumatically as is most common in veterinary patients. B) Thoracic cavity on necropsy contains approximately 2 liters of serosanguinous fluid. The liver (left) is pale and friable with an increased lobular pattern. C) Heart and pericardium on necropsy examination. The epicardium of ventricles and auricles were multifocally thickened and irregular with fibrous material and white to tan pyogranulomatous nodules.
FIGURE 3. Heart: The pericardium is markedly thickened with severe diffuse pyogranulomatous inflammation with multiple GMS (Grocott’s Methenamine silver stain)-positive fungal hyphae (insert:Aspergillus citrinoterreus ).
FIGURE 4. Aspergillus citrinoterreus (UTHSCSA DI20-341). A-C. Colonies on MEA, 7d at 25, 30 and 37 °C. D-E. Light micrographs of vesicles and accessory conidia at 7d.
FIGURE 5. A maximum likelihood tree constructed from combined sequences of partial BenA and CaM showing the relationship of isolate UTHSCSA DI20-341 with representative species in theAspergillus section Terrei. Values at the nodes are Bayesian posterior probability (BI) > 0.95 (right) and bootstrap (BS) > 70% (left). Ex-type cultures are marked with a T. Branches with double-bars are truncated two-fold.
FIGURE 6. Maximum likelihood trees from sequences of individual genesBenA and CaM showing the relationship of isolate UTHSCSA DI20-341 with representative species in the Aspergillus sectionTerrei . Values at the nodes are Bayesian posterior probability (BI) > 0.95 (right) and bootstrap (BS)> 70% (left). Ex-type cultures are marked with aT. Branches with double-bars are truncated two-fold.