Common garden experiment: adaptive variation of leaf trichomes and their relationships with stomatal traits
Moreover, we collected the seeds of 15 of 44 sampling sites in 2008, which were grown in a common garden. The common garden located in Shanghai at 4 m above sea-level, 31°02′N and 121°26′E, where MAT and MAP was 15.5 °C and 1149.8 mm, respectively. Different populations in the common garden followed a randomized complete block design (RCBD), where there were five replicates for each population. Leaf samples in the common garden were collected in 2011. For each replicate, three healthy seedlings were selected, and at least 10 healthy and mature leaves were collected from the middle of the south-facing crown.