2.2.2 (e) Rv2615c protein trigger the activation of Caspase9 in
THP-1 macrophages
Initiator Caspase 9 activation is necessary for the development of the
apoptosome complex and the subsequent activation of executioner Caspases
3 and 7, which results in apoptotic cell death. To check the engagement
of initiator Caspase9 with respect to apoptosis induction by selected
recombinant protein, western blot assessment of the total cell lysate
from THP-1 macrophages post-stimulation with Rv2615c protein was done to
determine if initiator Caspase9 was activated for the induction of
apoptosis in response to Rv2615c. Levels of activated Caspase9 was
quantified using ImageJ and revealed to be increased in response to
Rv2615c stimulation relative to unstimulated cells and LPS stimulation
(Fig 2a and 2b).