Rv2615c protein (pink color) showed high affinity for CARD domain of both APAF1 (orange color) and Caspase9 (blue color) and establishes interaction via its CARD-like domain. The binding affinity of Rv2615c for CARD domain of both APAF1 and Caspase9 was much higher than the positive control included in the study involving interaction of APAF1 CARD and Caspase9 CARD. However, Rv2615c showed low binding affinity for inactive form of APAF1 ADP implicating its preference for active APAF1 Apoptosome complex.