2.3. (c) Rv2615c leads to up-regulation of downstream effector molecule- MyD88 and NF-ƙB
RT-PCR evaluation of cDNA from isolated mRNA of unstimulated and Rv2615c protein/LPS stimulated THP-1 macrophages revealed an increase in fold change in MyD88 gene expression after 24h of stimulation and in NF-ƙB gene expression after 24 hours and 48 hours of stimulation. When compared to unstimulated cells, Rv2615c protein stimulation led to approximately ~2.6-fold increase and LPS stimulation led to approximately ~3-fold increase in the expression of MyD88 gene respectively (Fig 3d). Expression of NFκB was observed to be approximately ~4-fold increase in response to Rv2615c and approximately ~2 to 4-fold increase in response to LPS after 24 and 48hrs of stimulation as compared to unstimulated cells (Fig 3e).