MD simulations for 100 ns was performed for Rv2615c protein docked with APAF1 CARD, Caspase9 CARD and mutant Rv2615c protein docked with APAF1 CARD, Caspase9 CARD. Docked complex of APAF1 CARD and Caspase9 CARD was included as positive (a) Root mean square deviation for complex backbone with x axis denoting time in ns and y axis denoting RMSD in nm (b) and (c) Root mean square fluctuation of backbone for control (APAF1 CARD and Caspase9 CARD) and other complexes with x axis denoting atom index of complex and y axis denoting RMSF in nm.