THP-1 macrophages were unstimulated or stimulated with LPS/Rv2615c protein and for 16h, 24h and 48h were analysed for release of a) TNF-α and b) IL-1β in cell culture supernatants c) TNF-α release was also estimated in supernatant blocked with Anti-TLR4 antibody prior to stimulation. ELISA was done according to manufacturer’s instructions. Graphs were plotted for different time points with cytokine release in pg/ml on y axis, controls, and the protein Rv2615c on the x axis. The findings of the statistical analysis, which used the Student’s t test, are shown as Mean ± SEM for three separate experiments where * represents comparison with unstimulated cells and *P<0.05, **P<0.01, ***P<0.001.