2.1.1 Rv2615c is an antigenic protein predicted to be involved
in host cell death pathways
Vaxijen 2.0 server predicted Rv2615c to be a probable antigen with high
score of 1.73. 5 models were generated for secondary structure
prediction using I-TASSER server which were further subjected for energy
minimization in Chiron server. Model 1 was selected based on the C score
of -3.06 in I-TASSER. Ramachandran plot for Model 1 showed 97.5%
residues were present in favoured and generously allowed region while
only 2.5% residues were present in disallowed region. Errat score was
86.67 for Model 1 and Verify3D score was 81.13.
I-TASSER predictions related to functional role strongly pointed the
involvement of Rv2615c in host cell death pathways. Three top predicted
structural analogs and Gene ontology templates for Rv2615c were those of
Apoptosome complex (PDB ID: 5JUL, 3IZ8, 1VT4) and Apoptosome-ProCaspase9
CARD complex (PDB ID: 3IYT, 3IZA). Consensus GO terms for biological
process and molecular functions were – GO: 0006917 (apoptotic process)
and GO: 006919, GO: 0043028, GO: 0016505 (apoptotic process involved in
activation of cysteine type endo-peptidase activity).