Multiple sequence alignment of 8(a) CARD-APAF1 and CARD-Caspase9 8(b) Rv2615c and CARD-Caspase9 8(c) Rv2615c and CARD-APAF1. All the crucial conserved or similar residues are denoted by asterisk (*). The common Leucine116 and Isoleucine117 residues in Rv2615c protein which were mutated to Alanine are denoted by red arrow.