3.3 In vitro cell contact toxicity of optimum SilMA
After the above procedures, the concentration of 20% was selected. The
cell contact toxicity test was operated to evaluate the
cytocompatibility of optimum SilMA. The fusiform adherent cells were
obviously observed 24 hours after cell seeding, as shown in
Fig.
6a-d. After 48 hours of co-culture with the samples, the morphology of
cells began to appear difference. The cells around the PCL and SilMA
grew well and showed a long shuttle clustered growth [Fig. 6(f, g)].
Compared with blank control (Fig. 6e), no matter in morphology or
density of cells, there was no obvious difference. However, there were
almost no adherent cells in the positive control group (Fig. 6h). This
result was more obvious after Giemsa staining, which can dye the nucleus
of the cells purple or blue purple, while the cytoplasm became pink
(Fig. 6i-k). In Fig. 6l, the remaining cells showed the round or
irregular shape, vacuoles were observed in the cytoplasm, and the nuclei
were pyknotic.