McSuSy Substrate Substrate Km (mM) Vmax (U/mg) Kcat (s-1) Kcat/Km (mM-1s-1)
Wild-type Wild-type UDP 0.13±0.02 10.39±0.33 17.32±0.55 133.23
Sucrose 90.10±19.38 10.56±0.71 17.6±1.18 0.20
S31D S31D UDP 0.09±0.02 11.38±0.43 18.97±0.72 206.20
Sucrose 70.18±19.84 13.44±0.93 22.4±1.55 0.32
S31D/K684T/N685D S31D/K684T/N685D UDP 0.49±0.10 10.22±0.62 17.04±1.03 34.77
Sucrose 59.34±12.88 10.24±0.61 17.07±1.02 0.29