Our combined data demonstrate that OBs and g-exonemes are different types of vesicles, which discharge at different time points post-activation with the exocytosis of the OBs preceding discharge of the g-exonemes. Further, we showed that exocytosis of the g-exonemes, but not of the OBs, is sensitive to the calcium chelator BAPTA-AM, indicating its dependency on intracellular calcium.