The PPLP2-GFP-BirA parasite line was generated by using vector pARL-pffnpa -GFP-BirA (). The full-length gene was amplified via PCR using the respective primers (for primer sequences, see Table S4). Ligation of insert and vector backbone was mediated by KpnI and AvrII restriction sites. Transfection of the construct was carried out as described above. Episomal presence of the construct was checked by using the following primers: PPLP2-BirA-KpnI-FP and pGREP RP (for primer sequences, see Table S4).