Figure 4 . Chromatograms from AEX processes employing the following: (A–B) an isocratic wash containing 20 mM BTP, 0.001% (w/v) Pluronic F-68, and 206.5 mM TEA-Ac at pH 9 over 10 CVs, and a LGE containing 20 mM BTP, 0.001% (w/v) Pluronic F-68, 2 mM MgCl2, and 25–182.5 mM NaCl at pH 9 over 30 CVs; (C–D) an isocratic wash containing 20 mM BTP, 0.001% (w/v) Pluronic F-68, 206.5 mM TEA-Ac at pH 9 over 10 CVs, a second isocratic wash containing 20 mM BTP, 0.001% (w/v) Pluronic F-68, 2 mM MgCl2, and 25 mM NaCl at pH 9 over 10 CVs, and an isocratic elution containing 20 mM BTP, 0.001% (w/v) Pluronic F-68, 2 mM MgCl2, 116.7 mM NaCl at pH 9 over 10 CVs. (B) and (D) are magnified images of empty (left panel) and full-capsid peaks (right panel) from chromatograms (A) and (C), respectively. Blue and red traces correspond to A280 and A254, respectively. The dotted purple traces and dashed green traces correspond to the volumetric fraction of running buffer B pumped through the system and the solution conductivity, respectively.