2.11. Statistical analysis
 
All experiments were performed at least three times and a minimum of 2 bio-chips/inserts/cocultures were performed in each experiment (N = 3 experiments and n = 6 HepG2/C3a biochips, SK-HEP-1 inserts and HepG2/C3a/SK-HEP-1 cocultures). Data are presented as means ± standard deviations (SD) of the 6 replicates (for RTqPCR assays, only 3 replicates from 3 different experiments were used). To determine statistical differences, a one-way ANOVA test and unpaired t-test were performed using GraphPad software (Prism 8). Data with P values < 0.05 were identified as statistically significant and highlighted in the figures.