3.2. Define key residues by conservation and coevolution analysis
Conservation scores were calculated for each residue of DAADH using
ConSurf webserver (Fig. S1). Conservation analysis indicated that key
residues which maintain the interfacial interactions are more conserved
than others during the evolution (Table 1) [24].
Evolutionary coupling reflect the network of interactions between
residue pairs based on their evolutionary information in the
family[13]. Several co-evolved residues pairs are identified by
co-evolution analysis of dimeric interfacial interactions (Table 2).
Co-evolutionary analysis of protein residues can reveal the underlying
remote interactions of amino acid residues (Fig. 3A). Therefore, several
co-evolved residues pairs which are selected for mutation to enhance the
subunits interaction (Fig. 3B).