3.2. Define key residues by conservation and coevolution analysis
Conservation scores were calculated for each residue of DAADH using ConSurf webserver (Fig. S1). Conservation analysis indicated that key residues which maintain the interfacial interactions are more conserved than others during the evolution (Table 1) [24].
Evolutionary coupling reflect the network of interactions between residue pairs based on their evolutionary information in the family[13]. Several co-evolved residues pairs are identified by co-evolution analysis of dimeric interfacial interactions (Table 2). Co-evolutionary analysis of protein residues can reveal the underlying remote interactions of amino acid residues (Fig. 3A). Therefore, several co-evolved residues pairs which are selected for mutation to enhance the subunits interaction (Fig. 3B).